Fermentative Hydrogen Production from Lignocellulose by Mesophilic Clostridium populeti FZ10 Newly Isolated from Microcrystalline Cellulose-Acclimated Compost

Screening new Clostridium strains that can efficiently utilize lignocellulose to produce hydrogen is extremely important for dark fermentative production. In this study, a mesophilic hydrogen-producing bacterium, identified as populeti FZ10, was newly isolated from compost acclimated by microcrystalline cellulose. The strain could various cellulosic substrates. performances of production cellulose (MCC) and corn stalk (CS) were especially investigated. maximum yield rate MCC 177.5 ± 4.8 mL/g 7.7 0.2 mL·g−1·h−1, respectively. Furthermore, scanning electron microscopy (SEM) images showed the structure CS destroyed after fermentation, which be attributed presence exoglucanase, endoglucanase, β-glucosidase xylanase produced FZ10. 92.5 3.7 5.9 mL·g−1·h−1,respectively, with degradation 47.2 2.3% hemicellulose 58.1 2.0%. This study demonstrates FZ10 an ideal candidate directly converting into biohydrogen under conditions. discovery C. has special significance in field bioenergy.